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KMID : 1094720180230020238
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Biotechnology and Bioprocess Engineering
2018 Volume.23 No. 2 p.238 ~ p.245
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Comparison of Trophic Modes to Maximize Biomass and Lipid Productivity of Micractinium inermum NLP-F014
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Park Seong-Hwan
Kim Jeong-Mi
Park Young-Hyun
Kim Sun-Ah
Cho Sun-Ja
Yu Jae-Chul
Kang Chang-Min
Lee Tae-Ho
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Abstract
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An optimum trophic mode condition was investigated to maximize biomass and lipid productivity of Micractinium inermum NLP-F014, which grown successfully in blended wastewater medium. In this study, four trophic modes were used, including photoautotrophic, photoheterotrophic, heterotrophic and mixotrophic modes. Mixotrophic mode showed the highest biomass and lipid productivity. However, a high concentration of organics resulted the negative effect on the growth of M. inermum NLP-F014. Mixotrophic cultivation using glucose below 500 mg/L was able to produce maximum biomass productivity up to 0.90 ± 0.03 g/L/day as well as maximum lipid productivity up to 129.31 ± 0.10 mg/L/day. From lipid analysis on mixotrophic mode using glucose, the major fatty acids are oleic acid (C18:1), linoleic acid (C18:2) and palmitic acid (C16:0). These results suggest that mixotrophic mode cultivation with wastewater containing chemical oxygen demand (COD) below 500 mg/L could be applicable for biodiesel production of M. inermum NLP-F014.
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KEYWORD
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microalgae, trophic modes, mixotrophic mode, biomass productivity, lipid productivity
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